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Gibson assembly: an easy way to clone potyviral full-length infectious cDNA clones expressing an ectopic VPg
BACKGROUND: Approaches to simplify and accelerate the construction of full-length infectious cDNA clones for plant potyviruses have been described, based on cloning strategies involving in vitro ligation or homologous recombination in yeast. In the present study, we developed a faster and more effic...
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| Publicado no: | Virol J |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4475333/ https://ncbi.nlm.nih.gov/pubmed/26070311 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12985-015-0315-3 |
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