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Highly Amyloidogenic Two-chain Peptide Fragments Are Released upon Partial Digestion of Insulin with Pepsin

Proteases play a well recognized role in the emergence of highly aggregation-prone protein fragments in vivo, whereas in vitro limited proteolysis is often employed to probe different phases of amyloidogenic pathways. Here, we show that addition of moderate amounts of pepsin to acidified bovine insu...

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Pubblicato in:J Biol Chem
Autori principali: Piejko, Marcin, Dec, Robert, Babenko, Viktoria, Hoang, Agnieszka, Szewczyk, Monika, Mak, Paweł, Dzwolak, Wojciech
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Biochemistry and Molecular Biology 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4358232/
https://ncbi.nlm.nih.gov/pubmed/25586185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.608844
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