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Observation of Superoxide Production During Catalysis of Bacillus subtilis Oxalate Decarboxylase at pH4
This contribution describes the trapping of the hydroperoxyl radical at a pH of 4 during turnover of wild-type oxalate decarboxylase and its T165V mutant using the spin trap BMPO. Radicals were detected and identified by a combination of EPR and mass spectrometry. Superoxide, or its conjugate acid,...
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| Publicado no: | Free Radic Biol Med |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4355160/ https://ncbi.nlm.nih.gov/pubmed/25526893 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2014.12.012 |
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