Observation of Superoxide Production During Catalysis of Bacillus subtilis Oxalate Decarboxylase at pH4

This contribution describes the trapping of the hydroperoxyl radical at a pH of 4 during turnover of wild-type oxalate decarboxylase and its T165V mutant using the spin trap BMPO. Radicals were detected and identified by a combination of EPR and mass spectrometry. Superoxide, or its conjugate acid,...

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Detaylı Bibliyografya
Yayımlandı:Free Radic Biol Med
Asıl Yazarlar: Twahir, Umar T., Stedwell, Corey N., Lee, Cory T., Richards, Nigel G. J., Polfer, Nicolas C., Angerhofer, Alexander
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2014
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Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4355160/
https://ncbi.nlm.nih.gov/pubmed/25526893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2014.12.012
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