Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Respirometric profiling of isolated mitochondria is commonly used to investigate electron transport chain function. We describe a method for obtaining samples of human Vastus lateralis, isolating mitochondria from minimal amounts of skeletal muscle tissue, and plate based respirometric profiling usi...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Bharadwaj, Manish S., Tyrrell, Daniel J., Lyles, Mary F., Demons, Jamehl L., Rogers, George W., Molina, Anthony J. A.
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2015
Teme:
Medicine
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354623/
https://ncbi.nlm.nih.gov/pubmed/25741892
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52350
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