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Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

The generation of patient-specific cell lines represents an invaluable tool for diagnostic or translational research, and these cells can be collected from skin or muscle biopsy tissue available during the patient’s diagnostic workup. In this protocol, we describe a technique for live cell isolation...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Robin, Jerome D., Wright, Woody E., Zou, Yaqun, Cossette, Stacy C., Lawlor, Michael W., Gussoni, Emanuela
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354544/
https://ncbi.nlm.nih.gov/pubmed/25651101
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52307
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