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Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

The generation of patient-specific cell lines represents an invaluable tool for diagnostic or translational research, and these cells can be collected from skin or muscle biopsy tissue available during the patient’s diagnostic workup. In this protocol, we describe a technique for live cell isolation...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Robin, Jerome D., Wright, Woody E., Zou, Yaqun, Cossette, Stacy C., Lawlor, Michael W., Gussoni, Emanuela
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354544/
https://ncbi.nlm.nih.gov/pubmed/25651101
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52307
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