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A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl

The serine protease Granzyme B (GzmB) mediates target cell apoptosis when released by cytotoxic T lymphocytes (CTL) or natural killer (NK) cells. GzmB is the most studied granzyme in humans and mice and therefore, researchers need specific and reliable tools to study its function and role in pathoph...

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Vydáno v:J Vis Exp
Hlavní autoři: Hagn, Magdalena, Sutton, Vivien R., Trapani, Joseph A.
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354391/
https://ncbi.nlm.nih.gov/pubmed/25489668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52419
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