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Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
Prototrophic bacteria grow on M-9 minimal salts medium supplemented with glucose (M-9 medium), which is used as a carbon and energy source. Auxotrophs can be generated using a transposome. The commercially available, Tn5-derived transposome used in this protocol consists of a linear segment of DNA c...
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| Publicado no: | J Vis Exp |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4353396/ https://ncbi.nlm.nih.gov/pubmed/25408097 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51934 |
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