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Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Understanding the mechanisms by which molecular motors coordinate their activities to transport vesicular cargoes within neurons requires the quantitative analysis of motor/cargo associations at the single vesicle level. The goal of this protocol is to use quantitative fluorescence microscopy to cor...

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Библиографические подробности
Опубликовано в: :J Vis Exp
Главные авторы: Neumann, Sylvia, Campbell, George E., Szpankowski, Lukasz, Goldstein, Lawrence S.B., Encalada, Sandra E.
Формат: Artigo
Язык:Inglês
Опубликовано: MyJove Corporation 2014
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4353387/
https://ncbi.nlm.nih.gov/pubmed/25406537
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52029
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