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Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
Here we present a protocol for preparation of acute brain slices. This procedure is a critical element for electrophysiological patch-clamp experiments that largely determines the quality of results. It has been shown that omitting the cooling step during cutting procedure is beneficial in obtaining...
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| Pubblicato in: | J Vis Exp |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
MyJove Corporation
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4353380/ https://ncbi.nlm.nih.gov/pubmed/25406663 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52068 |
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