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Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
Here we present a protocol for preparation of acute brain slices. This procedure is a critical element for electrophysiological patch-clamp experiments that largely determines the quality of results. It has been shown that omitting the cooling step during cutting procedure is beneficial in obtaining...
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| Vydáno v: | J Vis Exp |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
MyJove Corporation
2014
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4353380/ https://ncbi.nlm.nih.gov/pubmed/25406663 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52068 |
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