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Exploring the folding pathway of green fluorescent protein through disulfide engineering
We have introduced two disulfide crosslinks into the loop regions on opposite ends of the beta barrel in superfolder green fluorescent protein (GFP) in order to better understand the nature of its folding pathway. When the disulfide on the side opposite the N/C-termini is formed, folding is 2× faste...
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| Publicado no: | Protein Sci |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BlackWell Publishing Ltd
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4353360/ https://ncbi.nlm.nih.gov/pubmed/25516354 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2621 |
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