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Toward Computationally Designed Self-Reporting Biosensors Using Leave-One-Out Green Fluorescent Protein
Leave-one-out green fluorescent protein (LOOn-GFP) is a circularly permuted and truncated GFP lacking the nth β-strand element. LOO7-GFP derived from the wild-type sequence (LOO7-WT) folds and reconstitutes fluorescence upon addition of β-strand 7 (S7) as an exogenous peptide. Computational protein...
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| Publicado no: | Biochemistry |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4939794/ https://ncbi.nlm.nih.gov/pubmed/26397806 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b00786 |
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