A reproducible method for the expansion of mouse CD8 + T lymphocytes

Murine adoptive CD8 + T-cell immunotherapy studies require the generation of large numbers of high viability CD8 + cells. Here we report a tissue culture protocol for the reliable expansion of CD8 + T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells wer...

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Detalhes bibliográficos
Publicado no:J Immunol Methods
Main Authors: Lewis, Mark D., de Leenheer, Evy, Fishman, Sigal, Siew, Lai Khai, Gross, Gideon, Wong, F. Susan
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2015
Assuntos:
Technical Note
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4352898/
https://ncbi.nlm.nih.gov/pubmed/25602136
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2015.01.004
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