The dilution effect and the importance of selecting the right internal control genes for RT-qPCR: a paradigmatic approach in fetal sheep

BACKGROUND: The key to understanding changes in gene expression levels using reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) relies on the ability to rationalize the technique using internal control genes (ICGs). However, the use of ICGs has become increasingly probl...

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Detalhes bibliográficos
Publicado no:BMC Res Notes
Main Authors: Xu, Huaisheng, Bionaz, Massimo, Sloboda, Deborah M, Ehrlich, Loreen, Li, Shaofu, Newnham, John P, Dudenhausen, Joachim W, Henrich, Wolfgang, Plagemann, Andreas, Challis, John RG, Braun, Thorsten
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4352295/
https://ncbi.nlm.nih.gov/pubmed/25881111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-015-0973-7
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