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Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA

Immunoaffinity enrichment of peptides coupled to targeted, multiple reaction monitoring-mass spectrometry (immuno-MRM) has recently been developed for quantitative analysis of peptide and protein expression. As part of this technology, antibodies are generated to short, linear, tryptic peptides that...

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Foilsithe in:Mol Cell Proteomics
Main Authors: Schoenherr, Regine M., Saul, Richard G., Whiteaker, Jeffrey R., Yan, Ping, Whiteley, Gordon R., Paulovich, Amanda G.
Formáid: Artigo
Teanga:Inglês
Foilsithe: The American Society for Biochemistry and Molecular Biology 2015
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4350033/
https://ncbi.nlm.nih.gov/pubmed/25512614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O114.043133
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