How Many proteins are Missed in Quantitative proteomics Based on Ms/Ms sequencing Methods?

Current bottom-up quantitative proteomics methods based on MS/MS sequencing of peptides are shown to be strongly dependent on sample preparation. Using cytosolic proteins from MCF-7 breast cancer cells, it is shown that protein pre-fractionation based on pI and MW is more effective than pre-fraction...

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Udgivet i:Proteomics Insights
Main Authors: Mulvey, Claire, Thur, Bettina, Crawford, Mark, Godovac-Zimmermann, Jasminka
Format: Artigo
Sprog:Inglês
Udgivet: 2010
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4340538/
https://ncbi.nlm.nih.gov/pubmed/25729266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4137/PRI.S5882
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