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Determination of the Absolute Number of Escherichia coli Membrane Vesicles That Catalyze Active Transport

Transport of vinylglycolate (2-hydroxy-3-butenoic acid) via the lactate transport system is the limiting step for covalent labeling of membrane vesicles prepared from E. coli ML 308-225. Thus, the rate and extent of vinylglycolate labeling is stimulated about 10-fold by ascorbate-phenazine methosulf...

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Main Authors: Short, S. A., Kaback, H. R., Kaczorowski, G., Fisher, J., Walsh, C. T., Silverstein, S. C.
格式: Artigo
語言:Inglês
出版: 1974
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC434034/
https://ncbi.nlm.nih.gov/pubmed/4612538
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