Total Reconstitution of Functionally Active 50S Ribosomal Subunits from Escherichia coli
Total reconstitution of 50S subunits from E. coli was achieved by a two-step incubation procedure. In the first step, 23S RNA, 5S RNA, and the total proteins from 50S subunits were incubated for 20 min at 40° in the presence of 4 mM Mg(++) and 400 mM NH(4)Cl. In the second step, the Mg(++) concentra...
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| Опубликовано в:: | Proc Natl Acad Sci U S A |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
1974
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433966/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4612527/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.12.4713 |
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