Qualification of a whole blood intracellular cytokine staining assay to measure mycobacteria-specific CD4 and CD8 T cell immunity by flow cytometry(☆)

BACKGROUND: Qualified or validated assays are essential in clinical trials. Short-term stimulation of whole blood and intracellular cytokine staining assay is commonly used to measure immunogenicity in tuberculosis vaccine clinical trials. Previously, the short-term stimulation process of whole bloo...

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Bibliographic Details
Published in:J Immunol Methods
Main Authors: Kagina, Benjamin M., Mansoor, Nazma, Kpamegan, Eloi P., Penn-Nicholson, Adam, Nemes, Elisa, Smit, Erica, Gelderbloem, Sebastian, Soares, Andreia P., Abel, Brian, Keyser, Alana, Sidibana, Mzwandile, Hughes, Jane E., Kaplan, Gilla, Hussey, Gregory D., Hanekom, Willem A., Scriba, Thomas J.
Format: Artigo
Language:Inglês
Published: 2014
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4339399/
https://ncbi.nlm.nih.gov/pubmed/25523923
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2014.12.003
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