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Preparation of cell-permeable Cre recombinase by expressed protein ligation
BACKGROUND: Protein transduction is safer than viral vector-mediated transduction for the delivery of a therapeutic protein into a cell. Fusion proteins with an arginine-rich cell-penetrating peptide have been produced in E. coli, but the low solubility of the fusion protein expressed in E. coli imp...
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| Опубликовано в: : | BMC Biotechnol |
|---|---|
| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2015
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4339299/ https://ncbi.nlm.nih.gov/pubmed/25888446 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-015-0126-z |
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