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Preparation of cell-permeable Cre recombinase by expressed protein ligation

BACKGROUND: Protein transduction is safer than viral vector-mediated transduction for the delivery of a therapeutic protein into a cell. Fusion proteins with an arginine-rich cell-penetrating peptide have been produced in E. coli, but the low solubility of the fusion protein expressed in E. coli imp...

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Detalhes bibliográficos
Publicado no:BMC Biotechnol
Main Authors: Lyu, Soo Kyung, Kwon, Hyockman
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4339299/
https://ncbi.nlm.nih.gov/pubmed/25888446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-015-0126-z
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