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Identification of the Chloramphenicol-Binding Protein in Escherichia coli Ribosomes by Partial Reconstitution

50S-derived cores were prepared by treatment of 50S subunits with 0.4 M Licl (0.4c core) and 0.8 M Licl (0.8c core), respectively. 0.4c cores bind chloramphenicol whereas 0.8c cores do not. The split proteins obtained during the transitions 0.4c → 0.8c were separated by DEAE-cellulose chromatography...

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Bibliografiske detaljer
Main Authors: Nierhaus, Dagmar, Nierhaus, Knud H.
Format: Artigo
Sprog:Inglês
Udgivet: 1973
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC433706/
https://ncbi.nlm.nih.gov/pubmed/4365366
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