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Identification of the Chloramphenicol-Binding Protein in Escherichia coli Ribosomes by Partial Reconstitution

50S-derived cores were prepared by treatment of 50S subunits with 0.4 M Licl (0.4c core) and 0.8 M Licl (0.8c core), respectively. 0.4c cores bind chloramphenicol whereas 0.8c cores do not. The split proteins obtained during the transitions 0.4c → 0.8c were separated by DEAE-cellulose chromatography...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Nierhaus, Dagmar, Nierhaus, Knud H.
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1973
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Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433706/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4365366/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.8.2224
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