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Proteins interacting with cloning scars: a source of false positive protein-protein interactions

A common approach for exploring the interactome, the network of protein-protein interactions in cells, uses a commercially available ORF library to express affinity tagged bait proteins; these can be expressed in cells and endogenous cellular proteins that copurify with the bait can be identified as...

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Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Banks, Charles A. S., Boanca, Gina, Lee, Zachary T., Florens, Laurence, Washburn, Michael P.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4336944/
https://ncbi.nlm.nih.gov/pubmed/25704442
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep08530
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