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Protein-protein interaction assays: eliminating false positive interactions

Many methods commonly used to identify and characterize interactions between two or more proteins are variations of the immobilized protein-protein interaction assay (for example, glutathione S-transferase (GST) pulldown and coimmunoprecipitation). A potential, and often overlooked, problem with the...

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Bibliografiske detaljer
Main Authors: Nguyen, Tuan N, Goodrich, James A
Format: Artigo
Sprog:Inglês
Udgivet: 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2730730/
https://ncbi.nlm.nih.gov/pubmed/16432524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth0206-135
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