Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

This manuscript illustrates a protocol for efficiently creating integration-free human induced pluripotent stem cells (iPSCs) from peripheral blood using episomal plasmids and histone deacetylase (HDAC) inhibitors. The advantages of this approach include: (1) the use of a minimal amount of periphera...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Hubbard, Jesse J., Sullivan, Spencer K., Mills, Jason A., Hayes, Brian J., Torok-Storb, Beverly J., Ramakrishnan, Aravind
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2014
Subjects:
Cellular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4335579/
https://ncbi.nlm.nih.gov/pubmed/25408260
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52009
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