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Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
This manuscript illustrates a protocol for efficiently creating integration-free human induced pluripotent stem cells (iPSCs) from peripheral blood using episomal plasmids and histone deacetylase (HDAC) inhibitors. The advantages of this approach include: (1) the use of a minimal amount of periphera...
Gardado en:
| Publicado en: | J Vis Exp |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2014
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4335579/ https://ncbi.nlm.nih.gov/pubmed/25408260 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52009 |
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