Recircularization and Autonomous Replication of a Sheared R-Factor DNA Segment in Escherichia coli Transformants
Controlled shearing of R-factor DNA leads to formation of fragments carrying an antibiotic resistance gene present on, but not expressed by, the intact R-factor. Transformation of CaCl(2)-treated E. coli by such fragments yields an autonomously replicating tetracycline-resistance plasmid (Tc6-5) tha...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1973
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433482/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4576014/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.5.1293 |
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