Recircularization and Autonomous Replication of a Sheared R-Factor DNA Segment in Escherichia coli Transformants

Controlled shearing of R-factor DNA leads to formation of fragments carrying an antibiotic resistance gene present on, but not expressed by, the intact R-factor. Transformation of CaCl(2)-treated E. coli by such fragments yields an autonomously replicating tetracycline-resistance plasmid (Tc6-5) tha...

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Publikašuvnnas:	Proc Natl Acad Sci U S A
Váldodahkkit:	Cohen, Stanley N., Chang, Annie C. Y.
Materiálatiipa:	Artigo
Giella:	Inglês
Almmustuhtton:	National Academy of Sciences 1973
Fáttát:	Biological Sciences: Biochemistry
Liŋkkat:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433482/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4576014/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.5.1293
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