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Use of DNA Polymerase I Primed by a Synthetic Oligonucleotide to Determine a Nucleotide Sequence in Phage f1 DNA

A sequence of 50 residues in f1 DNA has been determined by the extension of a chemically synthesized octadeoxyribonucleotide by Escherichia coli DNA polymerase I, with radioactive nucleoside triphosphates and f1 DNA template. The polymerized product was synthesized either in the presence of manganes...

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Detalhes bibliográficos
Main Authors: Sanger, F., Donelson, J. E., Coulson, A. R., Kössel, H., Fischer, D.
Formato: Artigo
Idioma:Inglês
Publicado em: 1973
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC433459/
https://ncbi.nlm.nih.gov/pubmed/4577794
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