Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells

[Image: see text] We demonstrate the evolution of the PylRS/tRNA(CUA) pair for genetically encoding photocaged cysteine. By characterizing the incorporation in Escherichia coli and mammalian cells, and the photodeprotection process in vitro and in mammalian cells, we establish conditions for rapid e...

Ful tanımlama

Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:J Am Chem Soc
Asıl Yazarlar: Nguyen, Duy P., Mahesh, Mohan, Elsässer, Simon J., Hancock, Susan M., Uttamapinant, Chayasith, Chin, Jason W.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Chemical Society 2014
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4333589/
https://ncbi.nlm.nih.gov/pubmed/24479649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja412191m
Etiketler: Etiketle
Etiket eklenmemiş, İlk siz ekleyin!

Benzer Materyaller