Genetically Encoded Optochemical Probes for Simultaneous Fluorescence Reporting and Light Activation of Protein Function with Two-Photon Excitation

[Image: see text] The site-specific incorporation of three new coumarin lysine analogues into proteins was achieved in bacterial and mammalian cells using an engineered pyrrolysyl-tRNA synthetase system. The genetically encoded coumarin lysines were successfully applied as fluorescent cellular probe...

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Bibliografski detalji
Izdano u:J Am Chem Soc
Glavni autori: Luo, Ji, Uprety, Rajendra, Naro, Yuta, Chou, Chungjung, Nguyen, Duy P., Chin, Jason W., Deiters, Alexander
Format: Artigo
Jezik:Inglês
Izdano: American Chemical Society 2014
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4333581/
https://ncbi.nlm.nih.gov/pubmed/25341086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja5055862
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