In Vitro Repression of the Transcription of gal Operon by Purified gal Repressor

We have studied the in vitro repression of gal mRNA synthesis by the gal repressor from Escherichia coli. By use of a four-step purification procedure involving chromatography on phosphocellulose, DEAE-cellulose, and an affinity resin, the gal repressor has been purified about 1600-fold from a crude...

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Bibliographic Details
Main Authors: Nakanishi, Shigetada, Adhya, Sankar, Gottesman, M. E., Pastan, Ira
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1973
Subjects:
Biological Sciences: Biochemistry
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC433252/
https://ncbi.nlm.nih.gov/pubmed/4346883
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