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In Vitro Repression of the Transcription of gal Operon by Purified gal Repressor

We have studied the in vitro repression of gal mRNA synthesis by the gal repressor from Escherichia coli. By use of a four-step purification procedure involving chromatography on phosphocellulose, DEAE-cellulose, and an affinity resin, the gal repressor has been purified about 1600-fold from a crude...

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Autors principals: Nakanishi, Shigetada, Adhya, Sankar, Gottesman, M. E., Pastan, Ira
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1973
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC433252/
https://ncbi.nlm.nih.gov/pubmed/4346883
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