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Requirement of Proteins S5 and S9 from 30S Subunits for the Ribosome-Dependent GTPase Activity of Elongation Factor G

During CsCl isopycnic centrifugation at 20 mM Mg(++), Escherichia coli 30S ribosomal subunits specifically lose proteins S1, S2, S3, S5, S9, S10, and S14. The resultant 30S core is unable to stimulate the GTPase activity of EF-G in the presence of 50S subunits. Activity could be restored to a small...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Marsh, Robert C., Parmeggiani, Andrea
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1973
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433204/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4346030/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.1.151
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