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Radioimmune determination of hypoxanthine phosphoribosyltransferase crossreacting material in erythrocytes of Lesch-Nyhan patients.

We have developed a sensitive radioimmunoassay capable of detecting and quantitating 20 ng of hypoxanthine phosphoribosyltransferase (EC 2.4.2.8; IMP:pyrophosphate phosphoribosyltransferase) protein. For this assay, hypoxanthine phosphoribosyltransferase from human erythrocytes was iodinated with 12...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Ghangas, G S, Milman, G
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1975
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433156/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1060095/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.72.10.4147
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