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Molecular beacon enabled purification of living cells by targeting cell-type specific mRNAs
Molecular beacons (MBs) are dual-labeled oligonucleotides that fluoresce only in the presence of complementary mRNA. The use of MBs to target specific mRNAs allows sorting of specific cells from a mixed cell population. In contrast to existing approaches that are limited by available surface markers...
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| 發表在: | Nat Protoc |
|---|---|
| Main Authors: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2014
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4326061/ https://ncbi.nlm.nih.gov/pubmed/25232937 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.154 |
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