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tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells

When oligonucleotide probes are microinjected into cells to image the distribution of RNAs, they are rapidly sequestered into the nucleus. As a result, it is difficult to detect mRNAs in the cytoplasm of living cells. We were able to overcome this process by attaching tRNA transcripts to the probes....

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Detalhes bibliográficos
Main Authors: Mhlanga, Musa M., Vargas, Diana Y., Fung, Cindy W., Kramer, Fred Russell, Tyagi, Sanjay
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1074395/
https://ncbi.nlm.nih.gov/pubmed/15809226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gki302
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