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Expression and partial purification of enzymatically active recombinant Ty1 integrase in Saccharomyces cerevisiae.

Integration of the Saccharomyces cerevisiae retrotransposon Ty1 into the genome requires Ty1 integrase (IN). Apparent functions of Ty1 IN are target-site determination, cleavage, and joining of donor strands. To further study the mechanism of Ty1 integration, an IN expression plasmid has been constr...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Moore, S P, Garfinkel, D J
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1994
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC43260/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8127892/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.5.1843
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