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Affinity labeling of the ribosomal decoding site with an AUG-substrate analog.
The trinucleotide AUG was condensed at the 5'-end with N-bromoacetyl-p-aminophenylphosphate. This bromoactylated AUG analog reacted irreversibly with the mRNA binding site of Escherichia coli 70S ribosomes. After reaction of 70S ribosomes with the AUG analog, labeled 30S subunits could be isola...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1975
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC432565/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1093183/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.72.4.1505 |
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