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Design and characterization of a photo-activatable hedgehog probe that mimics the natural lipidated form

We have generated a photoactivatable form of sonic hedgehog protein by modifying the N-terminal cysteine with the heterobifunctional photocrosslinker 4-maleimidobenzophenone (Bzm). The Bzm modification on ShhN imparted a significant increase in activity as assessed in the C3H10T1/2 functional assay...

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Detalhes bibliográficos
Publicado no:Arch Biochem Biophys
Main Authors: House, Alan J., Daye, Laura R., Tarpley, Michael, Addo, Kezia, Lamson, David S., Parker, Margie K., Bealer, Warren E., Williams, Kevin P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4322941/
https://ncbi.nlm.nih.gov/pubmed/25529135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2014.12.014
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