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Design and characterization of a photo-activatable hedgehog probe that mimics the natural lipidated form
We have generated a photoactivatable form of sonic hedgehog protein by modifying the N-terminal cysteine with the heterobifunctional photocrosslinker 4-maleimidobenzophenone (Bzm). The Bzm modification on ShhN imparted a significant increase in activity as assessed in the C3H10T1/2 functional assay...
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| Опубликовано в: : | Arch Biochem Biophys |
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| Главные авторы: | , , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2014
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4322941/ https://ncbi.nlm.nih.gov/pubmed/25529135 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2014.12.014 |
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