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A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules.

A protein-free nucleic acid preparation method for electron microscopy is described. The basic procedure is very similar to the classical protein monolayer spreading techniques. The carrier protein (usually cytochrome c) is replaced by benzyldimethylalkylammonium chloride. Both the hypophase method...

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Detalhes bibliográficos
Main Authors: Vollenweider, H J, Sogo, J M, Koller, T
Formato: Artigo
Idioma:Inglês
Publicado em: 1975
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC432245/
https://ncbi.nlm.nih.gov/pubmed/164030
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