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Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The glyoxalated nucleic acids are then subjected to elect...

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Detalhes bibliográficos
Main Authors: McMaster, G K, Carmichael, G G
Formato: Artigo
Idioma:Inglês
Publicado em: 1977
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC432050/
https://ncbi.nlm.nih.gov/pubmed/73185
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