Characterization of a common precursor to corticotropin and beta-lipotropin: cell-free synthesis of the precursor and identification of corticotropin peptides in the molecule.
mRNA was isolated from cultures of AtT-20/D-16v tumor cells and translated in a mRNA-dependent reticulocyte cell-free system. The corticotropin (ACTH) product was purified by a double-antibody immunoprecipitation procedure using antisera specific for the alpha(1-24) sequence of ACTH. The product is...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1977
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC432048/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/200934/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.11.4826 |
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