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Characterization of a common precursor to corticotropin and beta-lipotropin: cell-free synthesis of the precursor and identification of corticotropin peptides in the molecule.

mRNA was isolated from cultures of AtT-20/D-16v tumor cells and translated in a mRNA-dependent reticulocyte cell-free system. The corticotropin (ACTH) product was purified by a double-antibody immunoprecipitation procedure using antisera specific for the alpha(1-24) sequence of ACTH. The product is...

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Dettagli Bibliografici
Autori principali: Roberts, J L, Herbert, E
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1977
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC432048/
https://ncbi.nlm.nih.gov/pubmed/200934
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