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Tritium suicide selection of mammalian cell mutants defective in the transport of neutral amino acids.
Mouse lymphocytic cells of the established line GF-14 were allowed to accumulate intracellular 3H-labeled aminoisobutyric acid (AIB), frozen, and stored over liquid N2. After internal radiation had reduced survival to 1 in 10(4), survivors were plated and tested for their ability to transport AIB. O...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1977
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431983/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/200920/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.10.4549 |
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