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Construction of single chain Fv antibody against transferrin receptor and its protein fusion with alkaline phosphatase
AIM: To construct fusion protein of a single-chain antibody (scFv) against transferrin receptor (TfR) with alkaline phosphatase (AP). METHODS: The VH-linker-VL, namely scFv gene, was prepared by amplifying the VH and VL genes from plasmid pGEM-T-VH and pGEM-T-VL with splicing overlap extension polym...
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| Veröffentlicht in: | World J Gastroenterol |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Baishideng Publishing Group Inc
2005
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4316069/ https://ncbi.nlm.nih.gov/pubmed/15929188 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i21.3300 |
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