Construction of single chain Fv antibody against transferrin receptor and its protein fusion with alkaline phosphatase

AIM: To construct fusion protein of a single-chain antibody (scFv) against transferrin receptor (TfR) with alkaline phosphatase (AP). METHODS: The VH-linker-VL, namely scFv gene, was prepared by amplifying the VH and VL genes from plasmid pGEM-T-VH and pGEM-T-VL with splicing overlap extension polym...

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Vydáno v:World J Gastroenterol
Hlavní autoři: Yang, Dao-Feng, Zhu, Hui-Fen, Wang, Zhi-Hua, Shen, Guan-Xin, Tian, De-Ying
Médium: Artigo
Jazyk:Inglês
Vydáno: Baishideng Publishing Group Inc 2005
Témata:
Brief Reports
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4316069/
https://ncbi.nlm.nih.gov/pubmed/15929188
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i21.3300
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