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Construction of plasmids carrying the cI gene of bacteriophage lambda.

By techniques of recombination in vitro, we have constructed a plasmid bearing the repressor gene (cI) of bacteriophage lambda fused to the promoter of the lac operon. Strains carrying this plasmid overproduce lambda repressor. This functional cI gene was reconstituted by joining DNA fragments beari...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Backman, K, Ptashne, M, Gilbert, W
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1976
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431373/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1069307/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.73.11.4174
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